Journal: Frontiers in Immunology

Article Title: GPCRs overexpression and impaired fMLP-induced functions in neutrophils from chronic kidney disease patients

doi: 10.3389/fimmu.2024.1387566

Figure Lengend Snippet: CKD neutrophils overexpress intracellular AnxA1. Increased FPR2 expression in CKD neutrophils (A) . AnxA1 expression in neutrophils at intracellular (B) and membrane compartments (C) . AnxA1 secretion from neutrophils (D) and apoptotic neutrophils (E) cultured for 18 hours under serum starvation conditions. (Control n= 3–11 and CKD n= 3–36) **p<0.01; ***p<0.001 CKD vs Control. ns, Not significant.

Article Snippet: Patients’ samples were incubated with anti-AnxA1 goat antibody (1:100) (AF3770; R&D SYSTEM) and anti-myeloperoxidase rabbit antibody (1:50; Biodesign International, Inc., Maine, USA) overnight at 4°C, followed by incubation with anti-goat Alexa Fluor 467 and anti-rabbit Alexa Fluor 488 secondary antibodies (1:200; Invitrogen).

Techniques: Expressing, Membrane, Cell Culture, Control

Journal: Frontiers in Immunology

Article Title: GPCRs overexpression and impaired fMLP-induced functions in neutrophils from chronic kidney disease patients

doi: 10.3389/fimmu.2024.1387566

Figure Lengend Snippet: Infiltrate neutrophils in CKD kidney biopsies are AnxA + . H&E (A–E) and PAS staining (F–J) . Myeloperoxidase (MPO) and AnxA1 labeling (K–O) . Five CKD biopsies were selected and stained for morphological, fibrosis visualization, and immunofluorescence. Figure 5D – Insert: * immune cells infiltrated; MPO and AnxA1 labeling. The image is representative of at least three fields analyzed on each biopsy.

Article Snippet: Patients’ samples were incubated with anti-AnxA1 goat antibody (1:100) (AF3770; R&D SYSTEM) and anti-myeloperoxidase rabbit antibody (1:50; Biodesign International, Inc., Maine, USA) overnight at 4°C, followed by incubation with anti-goat Alexa Fluor 467 and anti-rabbit Alexa Fluor 488 secondary antibodies (1:200; Invitrogen).

Techniques: Staining, Labeling, Immunofluorescence